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VEGF Rabbit mAb#48707

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Product Detail

Product NameVEGF Rabbit mAb

Clone No.SP07-01

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsICC/IF, IHC, IP, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesFolliculostellate cell-derived growth factor antibody
Glioma-derived endothelial cell mitogen antibody
MGC70609 antibody
MVCD1 antibody
Vascular endothelial growth factor A antibody
vascular endothelial growth factor A121 antibody
vascular endothelial growth factor A165 antibody
vascular endothelial growth factor antibody
Vascular permeability factor antibody
VEGF A antibody
Vegf antibody
VEGF-A antibody
VEGF120 antibody
Vegfa antibody
VEGFA_HUMAN antibody
VPF antibody

Accession NoSwiss-Prot#:P15692

Uniprot P15692

Gene ID 7422;

Calculated MW43 kDa

Concentration1 mg/ml

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details


IHC:1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-VEGF antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human breast carcimoma tissue using anti-VEGF antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-VEGF antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-VEGF antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-VEGF antibody. Counter stained with hematoxylin.
ICC staining VEGF in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining VEGF in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining VEGF in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining VEGF in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with VEGF antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody

The onset of angiogenesis is believed to be an early event in tumorigenesis and may facilitate tumor progression and metastasis. Several growth factors with angiogenic activity have been described. These include fibroblast growth factors (FGFs), platelet derived growth factor (PDGF) and vascular endothelial growth factor (VEGF). VEGF is a dimeric glycoprotein with structural homology to PDGF. Several variants of VEGF have been described that arise by alternative mRNA splicing. It has been speculated that VEGF may function as a tumor angiogenesis factor in vivo because the expression pattern of VEGF is consistent with a role in embryonic angiogenesis. VEGF mRNA is formed in some primary tumors, VEGF is produced by tumor cell lines in vitro and VEGF mitogenic activity appears to be restricted to endothelial cells. A member of the PDGF receptor family, Flt, has been identified as a high-affinity receptor for VEGF.

If you have published an article using product 48707, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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