• Product NameKRT14 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total KRT14 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to residues near the C terminal of human Keratin, type I cytoskeletal 14
• Target NameKRT14
• Alternative Names    K14; NFJ; CK14; EBS3; EBS4
• Accession No. Swiss-Prot#: P02533
  NCBI Gene ID: 3861
  Gene Accssion: NP_000517
• UniprotP02533
• Gene ID3861;
• SDS-PAGE MW 52kd
• Concentration 0.6mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:25-1:100

This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11.

• Gel: 10%SDS-PAGE
  Lysates (from left to right): Mouse thymus tissue
  Amount of lysate: 30ug per lane
  Primary antibody: 1/300 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 3 seconds
• Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue using #36794 at dilution 1/25.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting