• Product Nameglyoxalase I antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationPurified by antigen-affinity chromatography.
• ApplicationsWB IHC IF
• Species ReactivityHu
• Immunogen TypeRecombinant protein
• Immunogen DescriptionRecombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 171 of glyoxalase I
• Target Nameglyoxalase I
• Accession No. Swiss-Prot:Q04760
  Gene ID:2739
• UniprotQ04760
• Gene ID2739;
• Concentration 1mg/ml
• Formulation Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

• Predicted MW: 21kd
  Western blotting: 1:500-1:3000
  Immunohistochemistry: 1:100-1:250
  Immunofluorescence: 1:100-1:200
  

The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere. [provided by RefSeq]

• Sample(30ug whole cell lysate)
  A: 293T
  B: A431
  C: H1299
  D: HeLa S3
  E: Hep G2
  F: MOLT4
  G: Raji
  12% SDS PAGE
  Primary antibody diluted at 1: 1000
• Immunohistochemical analysis of paraffin-embedded OVCA, using GLO1 antibody at 1: 100 dilution.
• Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using glyoxalase I antibody at 1: 200 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting