• Product NameTRAF7 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total TRAF7 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to a region derived from internal residues of human TNF receptor-associated factor 7, E3 ubiquitin protein ligase
• Target NameTRAF7
• Alternative Names    RFWD1; RNF119
• Accession No. Swiss-Prot#: Q6Q0C0
  NCBI Gene ID: 84231
  Gene Accssion: NP_115647.2
• UniprotQ6Q0C0
• Gene ID84231;
• SDS-PAGE MW 67kd
• Concentration 0.7mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:15-1:50

Tumor necrosis factor (TNF; see MIM 191160) receptor-associated factors, such as TRAF7, are signal transducers for members of the TNF receptor superfamily (see MIM 191190). TRAFs are composed of an N-terminal cysteine/histidine-rich region containing zinc RING and/or zinc finger motifs; a coiled-coil (leucine zipper) motif; and a homologous region that defines the TRAF family, the TRAF domain, which is involved in self-association and receptor binding.

• Gel: 8%SDS-PAGE
  Lysate: 40ug Jurkat cell
  Primary antibody: 1/300 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 2 minutes
• Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue using #37054 at dilution 1/20.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting