• Product NamePRKD1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total PRKD1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to residues near the C terminal of human protein kinase D1
• Target NamePRKD1
• Alternative Names    PKD; PKCM; PRKCM; PKC-MU
• Accession No. Swiss-Prot#: Q15139
  NCBI Gene ID: 5587
  Gene Accssion: NP_002733
• UniprotQ15139
• Gene ID5587;
• SDS-PAGE MW 102kd
• Concentration 3.3mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:50-1:200

PRKD1 is a serine/threonine kinase that regulates a variety of cellular functions, including membrane receptor signaling, transport at the Golgi, protection from oxidative stress at the mitochondria, gene transcription, and regulation of cell shape, motility, and adhesion.

• Gel: 6%SDS-PAGE
  Lysates (from left to right): 293T and NIH/3T3 cell
  Amount of lysate: 40ug per lane
  Primary antibody: 1/550 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 30 seconds
• Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue using #37125 at dilution 1/40.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting