• Product NamePRKAR1B Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total PRKAR1B protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to a region derived from internal residues of human Protein kinase, cAMP-dependent, regulatory, type I, beta
• Target NamePRKAR1B
• Alternative Names    PRKAR1
• Accession No. Swiss-Prot#: P31321
  NCBI Gene ID: 5575
  Gene Accssion: NP_001158230
• UniprotP31321
• Gene ID5575;
• SDS-PAGE MW 43kd
• Concentration 2.4mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:1000-1:5000
  Immunohistochemistry: 1:50-1:200

Cyclic AMP-dependent protein kinase A (PKA) is an essential enzyme in the signaling pathway of the second messenger cAMP. Through phosphorylation of target proteins, PKA controls many biochemical events in the cell including regulation of metabolism, ion transport, and gene transcription. The PKA holoenzyme is composed of 2 regulatory and 2 catalytic subunits and dissociates from the regulatory subunits upon binding of cAMP.

• Gel: 10%SDS-PAGE
  Lysates (from left to right): Human colon cancer tissue
  Amount of lysate: 40ug per lane
  Primary antibody: 1/1200 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 20 seconds
• Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue using #37215 at dilution 1/70.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting