• Product NameS1PR5 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total S1PR5 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to a region derived from internal residues of human Sphingosine-1-phosphate receptor 5
• Target NameS1PR5
• Alternative Names    EDG8; S1P5; Edg-8; SPPR-1; SPPR-2
• Accession No. Swiss-Prot#: Q9H228
  NCBI Gene ID: 53637
  Gene Accssion: NP_110387
• UniprotQ9H228
• Gene ID53637;
• SDS-PAGE MW 42kd
• Concentration 1.5mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  

The lysosphingolipid sphingosine 1-phosphate (S1P) regulates cell proliferation, apoptosis, motility, and neurite retraction. Its actions may be both intracellular as a second messenger and extracellular as a receptor ligand. S1P and the structurally related lysolipid mediator lysophosphatidic acid (LPA) signal cells through a set of G protein-coupled receptors known as EDG receptors. Some EDG receptors are S1P receptors; others are LPA receptors.

• Gel: 10%SDS-PAGE
  Lysates (from left to right): Human brain malignant glioma tissue and MCF7 cell
  Amount of lysate: 40ug per lane
  Primary antibody: 1/750 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 20 seconds

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting