• Product NameARFGEF2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total ARFGEF2 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to a region derived from internal residues of human ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited)
• Target NameARFGEF2
• Alternative Names    BIG2; PVNH2; dJ1164I10.1
• Accession No. Swiss-Prot#: Q9Y6D5
  NCBI Gene ID: 10564
  Gene Accssion: NP_006411
• UniprotQ9Y6D5
• Gene ID10564;
• SDS-PAGE MW 202kd
• Concentration 3.4mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:50-1:200

ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. The protein encoded by this gene is involved in the activation of ARFs by accelerating replacement of bound GDP with GTP and is involved in Golgi transport. It contains a Sec7 domain, which may be responsible for its guanine-nucleotide exchange activity and also brefeldin A inhibition.

• Gel: 6%SDS-PAGE
  Lysates (from left to right): Mouse heart tissue
  Amount of lysate: 50ug per lane
  Primary antibody: 1/200 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 5 minutes
• Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using #37349 at dilution 1/50.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting