• Product NameADPRHL2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total ADPRHL2 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to residues near the C terminal of human ADP-ribosylhydrolase like 2
• Target NameADPRHL2
• Alternative Names    ARH3
• Accession No. Swiss-Prot#: Q9NX46
  NCBI Gene ID: 54936
  Gene Accssion: NP_060295
• UniprotQ9NX46
• Gene ID54936;
• SDS-PAGE MW 39kd
• Concentration 0.8mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  

This gene encodes a member of the ADP-ribosylglycohydrolase family. The encoded enzyme catalyzes the removal of ADP-ribose from ADP-ribosylated proteins. This enzyme localizes to the mitochondria, in addition to the nucleus and cytoplasm. Poly(ADP-ribose) synthesized after DNA damage is only present transiently and is rapidly degraded by poly(ADP-ribose) glycohydrolase. Poly(ADP-ribose) metabolism may be required for maintenance of the normal function of neuronal cells. Generates ADP-ribose from poly-(ADP-ribose), but does not hydrolyze ADP-ribose-arginine, -cysteine, -diphthamide, or -asparagine bonds. Due to catalytic inactivity of PARG mitochondrial isoforms, ARH3 is the only PAR hydrolyzing enzyme in mitochondria.

• Gel: 12%SDS-PAGE
  Lysates (from left to right): Hela cell and mouse kidney tissue
  Amount of lysate: 40ug per lane
  Primary antibody: 1/500 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 2 minutes

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting