• Product NameASPN Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total ASPN protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to a region derived from internal residues of human asporin
• Target NameASPN
• Alternative Names    OS3; PLAP1; PLAP-1; SLRR1C
• Accession No. Swiss-Prot#: Q9BXN1
  NCBI Gene ID: 54829
  Gene Accssion: NP_001180264
• UniprotQ9BXN1
• Gene ID54829;
• SDS-PAGE MW 43kd
• Concentration 2.2mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  

This gene encodes a cartilage extracellular protein that is member of the small leucine-rich proteoglycan family. The encoded protein may regulate chondrogenesis by inhibiting transforming growth factor-beta 1-induced gene expression in cartilage. This protein also binds collagen and calcium and may induce collagen mineralization. Polymorphisms in the aspartic acid repeat region of this gene are associated with a susceptibility to osteoarthritis. Alternate splicing results in multiple transcript variants.

• Gel: 10%SDS-PAGE
  Lysates (from left to right): Hela and hepG2 cell
  Amount of lysate: 40ug per lane
  Primary antibody: 1/550 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 40 seconds

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting