• Product NameALOX15 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total ALOX15 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to residues near the C terminal of human arachidonate 15-lipoxygenase
• Target NameALOX15
• Alternative Names    15-lipooxygenase-1; 15-LOX; 15-LOX-1; 15LOX-1; ALOX15;
• Accession No. Swiss-Prot#: P16050
  NCBI Gene ID: 246
  Gene Accssion: NP_001127349
• UniprotP16050
• Gene ID246
• SDS-PAGE MW 75kd
• Concentration 1mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:1000-1:5000
  Immunohistochemistry: 1:50-1:200

Arachidonate 15-lipoxygenase?is an?enzyme?that in humans is encoded by the?ALOX15?gene. The ALOX15 gene maps to 17p13.3 in close proximity to the tumor-suppressor gene TP53. The ALOX15 gene product is implicated in antiinflammation, membrane remodeling, and cancer development/metastasis. Converts arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid. Also acts on C-12 of arachidonate as well as on linoleic acid.

• Gel: 10%SDS-PAGE
  Lysate: 40ug Hela cell
  Primary antibody: 1/1300 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 1 minute
• Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using #37409 at dilution 1/60.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting