• Product NameSMAD9 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total SMAD9 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to a region derived from internal residues of human SMAD family member 9
• Target NameSMAD9
• Alternative Names    PPH2; MADH6; MADH9; SMAD8; SMAD8A; SMAD8B
• Accession No. Swiss-Prot#: O15198
  NCBI Gene ID: 4093
  Gene Accssion: NP_001120689
• UniprotO15198
• Gene ID4093;
• SDS-PAGE MW 52kd
• Concentration 2.9mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:25-1:100

The protein encoded by this gene is a member of the SMAD family, which transduces signals from TGF-beta family members. The encoded protein is activated by bone morphogenetic proteins and interacts with SMAD4. Two transcript variants encoding different isoforms have been found for this gene.

• Gel: 8%SDS-PAGE
  Lysates (from left to right): Mouse heart tissue
  Amount of lysate: 40ug per lane
  Primary antibody: 1/290 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 3 minutes
• Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using #37419 at dilution 1/30.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting