• Product NameCENPE Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total CENPE protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to residues near the N terminal of human centromere protein E, 312kDa
• Target NameCENPE
• Alternative Names    KIF10; CENP-E; PPP1R61
• Accession No. Swiss-Prot#: Q02224
  NCBI Gene ID: 1062
  Gene Accssion: NP_001804
• UniprotQ02224
• Gene ID1062;
• SDS-PAGE MW 316kd
• Concentration 2.8mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:50-1:200

Centrosome-associated protein E is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle. Unlike other centrosome-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. CENPE is proposed to be one of the motors responsible for mammalian chromosome movement and/or spindle elongation.?

• Gel: 6%SDS-PAGE
  Lysates (from left to right): K562 and A431 cell
  Amount of lysate: 40ug per lane
  Primary antibody: 1/1400 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 1 minute
• Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue using #37482 at dilution 1/50.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting