• Product NameCLTC Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total CLTC protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to a region derived from internal residues of human clathrin, heavy chain (Hc)
• Target NameCLTC
• Alternative Names    Hc; CHC; CHC17; CLH-17; CLTCL2
• Accession No. Swiss-Prot#: Q00610
  NCBI Gene ID: 1213
  Gene Accssion: NP_004850
• UniprotQ00610
• Gene ID1213;
• SDS-PAGE MW 192kd
• Concentration 2.7mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:50-1:200

Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains.

• Gel: 6%SDS-PAGE
  Lysates (from left to right): NIH/3T3 and hela cell
  Amount of lysate: 40ug per lane
  Primary antibody: 1/300 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 40 seconds
• Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using #37491 at dilution 1/60.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting