• Product NameESPL1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total ESPL1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to a region derived from internal residues of human extra spindle pole bodies homolog 1 (S. cerevisiae)
• Target NameESPL1
• Alternative Names    ESP1; SEPA
• Accession No. Swiss-Prot#: Q14674
  NCBI Gene ID: 9700
  Gene Accssion: NP_036423
• UniprotQ14674
• Gene ID9700;
• SDS-PAGE MW 233kd
• Concentration 2mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:25-1:100

Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 or STAG2 in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 by ESPL1, or separase, which initiates the final separation of sister chromatids.

• Gel: 6%SDS-PAGE
  Lysate: 40ug Raji cell
  Primary antibody: 1/500 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 1 second
• Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using #37557 at dilution 1/40.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting