• Product NameGLS Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total GLS protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to residues near the C terminal of human glutaminase
• Target NameGLS
• Alternative Names    GAC; GAM; KGA; GLS1; AAD20
• Accession No. Swiss-Prot#: O94925
  NCBI Gene ID: 2744
  Gene Accssion: NP_055720
• UniprotO94925
• Gene ID2744;
• SDS-PAGE MW 73kd
• Concentration 1.7mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:25-1:100

This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants.

• Gel: 6%SDS-PAGE
  Lysate: 40ug HepG2 cell
  Primary antibody: 1/400 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 1 second
• Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue using #37597 at dilution 1/25.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting