• Product NameHES1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total HES1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to residues near the C terminal of human hes family bHLH transcription factor 1
• Target NameHES1
• Alternative Names    HHL; HRY; HES-1; bHLHb39
• Accession No. Swiss-Prot#: Q14469
  NCBI Gene ID: 3280
  Gene Accssion: NP_005515
• UniprotQ14469
• Gene ID3280;
• SDS-PAGE MW 30kd
• Concentration 0.7mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  

This protein belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box.

• Gel: 8%SDS-PAGE
  Lysates (from left to right): LoVo, Jurkat, HT-29 and MCF-7 cell
  Amount of lysate: 40ug per lane
  Primary antibody: 1/200 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 5 minutes

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting