• Product NameKDM4A Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total KDM4A protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to a region derived from internal residues of human lysine (K)-specific demethylase 4A
• Target NameKDM4A
• Alternative Names    JMJD2; JHDM3A; JMJD2A; TDRD14A
• Accession No. Swiss-Prot#: O75164
  NCBI Gene ID: 9682
  Gene Accssion: NP_055478
• UniprotO75164
• Gene ID9682;
• SDS-PAGE MW 121kd
• Concentration 2.6mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:50-1:200

This gene is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein containing a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor.?

• Gel: 6%SDS-PAGE
  Lysates (from left to right): NIH/3T3 cell and mouse liver tissue
  Amount of lysate: 40ug per lane
  Primary antibody: 1/650 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 5 minutes
• Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue using #37671 at dilution 1/40.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting