• Product NameERRFI1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total ERRFI1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to a region derived from internal residues of human ERBB receptor feedback inhibitor 1
• Target NameERRFI1
• Alternative Names    MIG6; RALT; MIG-6; GENE-33
• Accession No. Swiss-Prot#: Q9UJM3
  NCBI Gene ID: 54206
  Gene Accssion: NP_061821
• UniprotQ9UJM3
• Gene ID54206;
• SDS-PAGE MW 51kd
• Concentration 2.3mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:25-1:100

ERRFI1 is a cytoplasmic protein whose expression is upregulated with cell growth (Wick et al., 1995 [PubMed 7641805]). It shares significant homology with the protein product of rat gene-33, which is induced during cell stress and mediates cell signaling

• Gel: 8%SDS-PAGE
  Lysates (from left to right): Human brain malignant glioma tissue and HepG2 cell
  Amount of lysate: 40ug per lane
  Primary antibody: 1/400 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 5 minutes
• Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using #37731 at dilution 1/35.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting