• Product NameMOB1A Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total MOB1A protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to residues near the C terminal of human MOB kinase activator 1A
• Target NameMOB1A
• Alternative Names    MOB1; MATS1; Mob4B; C2orf6; MOBK1B; MOBKL1B
• Accession No. Swiss-Prot#: Q9H8S9
  NCBI Gene ID: 55233
  Gene Accssion: NP_060691
• UniprotQ9H8S9
• Gene ID55233;
• SDS-PAGE MW 25kd
• Concentration 1.3mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:25-1:100

MOB1 was first identified in yeast as a protein that binds to Mps with essential roles in the completion of mitosis and the maintenance of ploidy . Its?Drosophila?and mammalian homologs, Mats and MOB1, respectively, are involved in the Hippo signaling tumor suppressor pathway, which plays a critical role in organ size regulation and which has been implicated in cancer development .?

• Gel: 10%SDS-PAGE
  Lysates (from left to right): A375 and K562 cell, mouse liver tissue and Raji cell, Jurkat and Hela cell
  Amount of lysate: 40ug per lane
  Primary antibody: 1/200 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 10 seconds
• Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using #37737 at dilution 1/20.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting