• Product NameNEFM Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total NEFM protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to residues near the C terminal of human neurofilament, medium polypeptide
• Target NameNEFM
• Alternative Names    NFM; NEF3; NF-M
• Accession No. Swiss-Prot#: P07197
  NCBI Gene ID: 4741
  Gene Accssion: NP_005373
• UniprotP07197
• Gene ID4741;
• SDS-PAGE MW 102kd
• Concentration 1.1mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:25-1:100

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms.?

• Gel: 6%SDS-PAGE
  Lysates (from left to right): Human brain malignant glioma tissue
  Amount of lysate: 40ug per lane
  Primary antibody: 1/150 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 1 minutes
• Immunohistochemical analysis of paraffin-embedded Human brain tissue using #37760 at dilution 1/20.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting