• Product NameIVL Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total IVL protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to a region derived from internal residues of human involucrin
• Target NameIVL
• Alternative Names    INVO; Involucrin; IVL
• Accession No. Swiss-Prot#: P07476
  NCBI Gene ID: 3713
  Gene Accssion: NP_005538
• UniprotP07476
• Gene ID3713;
• SDS-PAGE MW 68kd
• Concentration 3.2mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:50-1:200

Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. This gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin.

• Gel: 6%SDS-PAGE
  Lysate: 40ug Hela cell
  Primary antibody: 1/200 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 20 seconds
• Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue using #37804 at dilution 1/50.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting