• Product NamePOU6F2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total POU6F2 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to a region derived from internal residues of human POU class 6 homeobox 2
• Target NamePOU6F2
• Alternative Names    WT5; WTSL; RPF-1
• Accession No. Swiss-Prot#: P78424
  NCBI Gene ID: 11281
  Gene Accssion: NP_001159490/P78424
• UniprotP78424
• Gene ID11281;
• SDS-PAGE MW 73kd
• Concentration 1 mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:2000
  Immunohistochemistry: 1:50~1:100

This gene encodes a member of the POU protein family characterized by the presence of a bipartite DNA binding domain, consisting of a POU-specific domain and a homeodomain, separated by a variable polylinker. The DNA binding domain may bind to DNA as monomers or as homo- and/or heterodimers, in a sequence-specific manner. The POU family members are transcriptional regulators, many of which are known to control cell type-specific differentiation pathways. This gene is a tumor suppressor involved in Wilms tumor (WT) predisposition.?

• Gel: 8%SDS-PAGELysate: 40 μgLane: Human placenta tissue lysatePrimary antibody: POU6F2 Antibody at dilution 1/800Secondary antibody: Goat anti rabbit IgG at 1/5000 dilutionExposure time: 3 minutes
• The image on the left is immunohistochemistry of paraffinembedded Human tonsil tissue using POU6F2 Antibody at dilution 1/50, on the right is treated with synthetic peptide.(Original magnification: ×200)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting