• Product NameKDM5A Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC ELISA
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total KDM5A protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to a region derived from internal residues of human lysine (K)-specific demethylase 5A
• Target NameKDM5A
• Alternative Names    RBP2; RBBP2; RBBP-2
• Accession No. Swiss-Prot#: P29375
  NCBI Gene ID: 5927
  Gene Accssion: NP_001036068/P29375
• UniprotP29375
• Gene ID5927;
• SDS-PAGE MW 192kd
• Concentration 1.2mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Immunohistochemistry: 1:50-1:300

  WB: 500-2000

This gene encodes a member of the Jumonji, AT-rich interactive domain 1 (JARID1) histone demethylase protein family. The encoded protein plays a role in gene regulation through the histone code by specifically demethylating lysine 4 of histone H3. The encoded protein interacts with many other proteins, including retinoblastoma protein, and is implicated in the transcriptional regulation of Hox genes and cytokines. This gene may play a role in tumor progression.

• The image on the left is immunohistochemistry of paraffinembedded Human liver cancer tissue using KDM5A Antibody at dilution 1/45, on the right is treated with synthetic peptide. (Original magnification: ×200)
• The image on the left is immunohistochemistry of paraffinembedded Human esophagus cancer tissue using KDM5A Antibody at dilution 1/45, on the right is treated with synthetic peptide. (Original magnification: ×200)
• Gel: 6%SDS-PAGE Lysate: 40 μg Lane 1-2: 231 cells, hela cells Primary antibody: KDM5AAntibody at dilution 1/200 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 2 minutes

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting