• Product NamePRAF2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total PRAF2 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to residues near the N terminal of human PRA1 domain family, member 2
• Target NamePRAF2
• Alternative Names    JM4
• Accession No. Swiss-Prot#: O60831
  NCBI Gene ID: 11230
  Gene Accssion: NP_009144/O60831
• UniprotO60831
• Gene ID11230;
• SDS-PAGE MW 19kd
• Concentration 2.4mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:25-1:100

JM4 (Jena-Muenchen 4), also known as PRAF2 (PRA1 domain family, member 2), is a 178 amino acid endosomal multi-pass membrane protein involved in vesicular trafficking and Endoplasmic reticulum/Golgi transport. As a member of the PRA1 family, JM4 contains four putative transmembrane (TM) domains, interacts with the CC chemokine receptor 5 (CCR5) and colocalizes with Calnexin in the ER and mannose 6-phosphate receptor (CD-MPR) in the Golgi apparatus.?

• Gel: 12%SDS-PAGE
  Lysates (from left to right): Human fetal brain tissue and A549 cell, hela and 293T cell
  Amount of lysate: 40ug per lane
  Primary antibody: 1/200 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 20 seconds
• Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue using #37884 at dilution 1/35.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting