• Product NameSIGLEC15 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB,IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total SIGLEC15 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide of human SIGLEC15
• Target NameSIGLEC15
• Alternative Names    CD33L3; HsT1361; SIGLEC-15
• Accession No. Swiss-Prot#: Q6ZMC9
  NCBI Gene ID: 284266
  Gene Accssion: NP_998767/Q6ZMC9
• UniprotQ6ZMC9
• Gene ID284266;
• Calculated MW 36kDa
• Concentration 0.9mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000  Immunofluorescence: 1:50-1:200  

Siglec-15 (sialic acid binding Ig-like lectin 15), also known as CD33L3, is a 328 amino acid single-pass type I membrane protein that contains one Ig-like C2-type domain and one Ig-like V-type domain. Expressed in dendritic and macrophage cells, Sinlec-15 interacts with DAP10 and DAP12 and binds to sialylated glycoproteins. The gene encoding Siglec-15 maps to human chromosome 18, which houses over 300 protein-coding genes and contains nearly 76 million bases.

• Gel: 8%SDS-PAGE Lysate: 40 ug Lane: Mouse brain tissue lysate Primary antibody: (SIGLEC15 Antibody) at dilution 1/800 Secondary antibody:(HRP-conjugated Goat anti rabbit IgG) at 1/5000 dilution Exposure time: 5 minutes
• The image on the left is immunohistochemistry of paraffinembedded Human colorectal cancer tissue using (SIGLEC15 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: 200)
• The image on the left is immunohistochemistry of paraffinembedded Human liver cancer tissue using (SIGLEC15 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: 200)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting