• Product NameCollagen III Mouse Monoclonal Antibody(Q76)
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.Q76
• PurificationAffinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHu Rt Ms
• SpecificityAntibody detects endogenous Collagen III protein.
• Target NameCollagen III
• Alternative Names    CO3A1; COL3A1; Collagen alpha-1(III) chain; collagen, fetal;
• Accession No. Swiss-Prot#:P02461
• UniprotP02461
• Gene ID1281;
• SDS-PAGE MW 138kd
• Concentration 1.0mg/ml
• Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB dilution: 1:1000
  IHC dilution:1:50-300
  IF dilution:1:200

Collagen alpha-1(III) chain is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with type I collagen.

• Western blot analysis of Hela cells, using #38065 diluted at 1:1,000.
• Immunofluorescence analysis of Mouse-liver tissue. 1,Collagen III Monoclonal Antibody(Q76)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
• Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,Collagen III Monoclonal Antibody(Q76) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
• Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1,Collagen III Monoclonal Antibody(Q76) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
• Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,Collagen III Monoclonal Antibody(Q76) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting