• Product Nameα Skeletal Muslce Actin Mouse Monoclonal Antibody(4B11)
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.4B11
• PurificationAffinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHu Rt Ms
• SpecificityAntibody detects endogenous α Skeletal Muscle Actin protein.
• Target Nameα Skeletal Muslce Actin
• Alternative Names    ACTA antibody, ACTA1 antibody, ACTC antibody, ACTC1 antibody, Actin alpha cardiac muscle antibody, ACTS antibody, Alpha actin 1 antibody, Cardiac muscle alpha actin 1 antibody, Skeletal muscle alpha actin 1 antibody
• Accession No. Swiss-Prot#:P68133
• UniprotP68133
• Gene ID58;
• SDS-PAGE MW 42kd
• Concentration 1.0mg/ml
• Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB dilution: 1:5000~1:10000
  IHC dilution:1:50-300IF dilution:1:200

Actin, alpha skeletal muscle is a protein that in humans is encoded by the ACTA1 gene. Actin alpha 1 which is expressed in skeletal muscle is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus.

• Western blot analysis of 1) Hela, 2) Mouse Brain tissue, 3) Rat Brain tissue, using #38090 diluted at 1:20,000.
• 1¡¢Input: Mouse Brain Tissue Lysate
  2¡¢IP product: IP dilute 1:200
  Western blot analysis: primary antibody: #38090 1:10,000
  Secondary antibody: Goat anti-Mouse IgG, Light chain specific, 1:5,000
• Immunofluorescence analysis of Human-liver-cancer tissue. 1, skeletal muscle actin Monoclonal Antibody(4B11)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
• Immunofluorescence analysis of Mouse-liver tissue. 1, skeletal muscle actin Monoclonal Antibody(4B11)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
• Immunofluorescence analysis of Rat-liver tissue. 1, skeletal muscle actin Monoclonal Antibody(4B11)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
• Immunohistochemical analysis of paraffin-embedded Rat-liver tissue. 1, skeletal muscle actin Monoclonal Antibody(4B11) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
• Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1, skeletal muscle actin Monoclonal Antibody(4B11) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting