• Product NameHistone H2B Mouse Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• PurificationAffinity purification using immunogen.
• ApplicationsWB,IF
• Species ReactivityHu Rt Ms
• SpecificityThe Histone H2B antibody can detects endogenous Histone H2B protein.
• Target NameHistone H2B
• Alternative Names    H2B GL105 antibody, H2B.1 antibody, Histone H2B type 1B antibody, HIST1H2BB antibody
• Accession No. Swiss-Prot#:P33778
• UniprotP33778
• Gene ID3018;
• SDS-PAGE MW 14kd
• Concentration 1.0mg/ml
• Formulation PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
• Storage Store at -20˚C

• WB dilution: 1:1000~1:3000
  
  IF dilution:1:200

Histone H2B is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells.

• Western blot analysis of 1) Hela, 2)3T3 , 3) Raw264.7, 4) Rat Brain, 5) Rat Kidney, using #38098 diluted at 1:2,000.
• Immunofluorescence analysis of Human-liver-cancer tissue. 1,Histone H2B Monoclonal Antibody(Mix)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
• Immunofluorescence analysis of Mouse-kidney tissue. 1,Histone H2B Monoclonal Antibody(Mix)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
• Immunofluorescence analysis of Rat-heart tissue. 1,Histone H2B Monoclonal Antibody(Mix)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting