• Product NameEGFR Mouse Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• PurificationAffinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHu
• SpecificityThe EGFR Mouse Monoclonal antibody detects endogenous EGFR proteins.
• Target NameEGFR
• Alternative Names    Receptor tyrosine-protein kinase ErbB-1; ERBB1
• Accession No. Swiss-Prot#:P00533
• UniprotP00533
• Gene ID1956;
• SDS-PAGE MW 170kd
• Concentration 1.0mg/ml
• Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB dilution: 1:1000
  
  IF dilution:1:100-200

The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades.

• Western blot analysis of Hela cell lysate, using #37996 diluted at 1:1,000.
• IF analysis of Hela with antibody (Left) and DAPI (Right) diluted at 1:100.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting