• Product NameCD23 Mouse Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.1.00E+09
• PurificationAffinity purification using immunogen.
• ApplicationsIHC,IF
• Species ReactivityHu Ms Rt
• SpecificityThe CD23 antibody detects endogenous CD23 proteins.
• Target NameCD23
• Alternative Names    Fc fragment of IgE, high affinity I, receptor for; Fc-epsilon RI-gamma; FCER1G; FCERG
• Accession No. Swiss-Prot#:P30273
• UniprotP30273
• Gene ID2207;
• SDS-PAGE MW 28,38,49kd
• Concentration 1.0mg/ml
• Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• IHC dilution: 1:200
  
  IF dilution:1:50-200

The CD23 antigen is the low affinity IgE Fc receptor, which is a 49 kDa protein with 38 and 28 kDa fragments. It is expressed on most mature, conventional B cells and can also be found on the surface of T cells, macrophages, platelets and EBV transformed B lymphoblasts. Expression of CD23 has been detected in neoplastic cells from cases of B cell chronic Lymphocytic leukemia. CD23 is expressed by B cells in the follicular mantle but not by proliferating germinal centre cells. CD23 is also expressed by eosinophils.

• IHC staining of paraffin-embedded Human tonsil tissue with CD23 mouse mAb£¨1E9£©diluted at 1:200.
• Immunofluorescence analysis of human-stomach tissue. 1,CD23 Monoclonal Antibody(1E9)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
• Immunofluorescence analysis of rat-lung tissue. 1,CD23 Monoclonal Antibody(1E9)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
• Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1,CD23 Monoclonal Antibody(1E9) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
• Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,CD23 Monoclonal Antibody(1E9) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
• Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,CD23 Monoclonal Antibody(1E9) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting