• Product Namealpha 1 Fetoprotein Mouse Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• PurificationAffinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHu
• SpecificityAFP Mouse Monoclonal antibody detects endogenous AFP protein.s
• Target Namealpha 1 Fetoprotein
• Alternative Names    AFP; Alpha-1-fetoprotein; Alpha-fetoglobulin; Alpha-fetoprotein; FETA; HPAFP
• Accession No. Swiss-Prot#:P02771
• UniprotP02771
• Gene ID174;
• SDS-PAGE MW 70kd
• Concentration 1.0mg/ml
• Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB dilution: 1:2000
  IHC dilution:1:50-300
  IF dilution:1:200

alpha-1-fetoprotein (AFP) is a protein that in humans is encoded by the AFP gene. The AFP gene is located on the q arm of chromosome 4 (4q25).AFP is a major plasma protein produced by the yolk sac and the liver during fetal development that is thought to be the fetal form of serum albumin. AFP binds to copper, nickel, fatty acids and bilirubin and is found in monomeric, dimeric and trimeric forms

• Western blot analysis of HepG2, using #38032 diluted at 1:2,000.
• Immunofluorescence analysis of Human-breast-cancer tissue. 1,AFP alpha 1 Fetoprotein Monoclonal Antibody(17C5)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
• Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1,AFP alpha 1 Fetoprotein Monoclonal Antibody(17C5) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting