• Product NameCaspase 9 Mouse Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• PurificationAffinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHu Rt Ms
• SpecificityAntibody detects endogenous Caspase 9 protein.
• Target NameCaspase 9
• Alternative Names    APAF-3; APAF3; apoptotic protease activating factor 3; Apoptotic protease Mch-6
• Accession No. Swiss-Prot#:P55211
• UniprotP55211
• Gene ID842;
• SDS-PAGE MW 46kd
• Concentration 1.0mg/ml
• Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB dilution: 1:1000~1:5000
  IP dilution: 1:200
  IHC dilution:1:50-300
  IF dilution:1:200

Caspase-9 is an initiator caspase, encoded by the CASP9 gene.Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).

• Western blot analysis of Hela cells, using #38034 diluted at 1£©1:2,000 2£©1:5,000
• Western blot analysis of 1£©Jurkat, 2) HepG2, 3) Mouse Heart, 4) Rat Heart, using #38034 diluted at 1:3,000.
• 1¡¢Input: Hela Cell Lysate
  2¡¢IP product: IP dilute 1:200
  Western blot analysis: primary antibody : #38034 1:1,000
  Secondary antibody: Goat anti-Mouse IgG, Light chain specific, 1:5,000
• Immunofluorescence analysis of Human-appendix tissue. 1,Caspase 9 Monoclonal Antibody(3-20)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
• Immunofluorescence analysis of Mouse-brain tissue. 1,Caspase 9 Monoclonal Antibody(3-20)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
• Immunofluorescence analysis of Rat-spleen tissue. 1,Caspase 9 Monoclonal Antibody(3-20)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
• Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1,Caspase 9 Monoclonal Antibody(3-20) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
• Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,Caspase 9 Monoclonal Antibody(3-20) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
• Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,Caspase 9 Monoclonal Antibody(3-20) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting