• Product NameCK17 Mouse Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• PurificationAffinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHu
• SpecificityCK17 Mouse Monoclonal antibody detects CK17 endogenous proteins.
• Target NameCK17
• SDS-PAGE MW 48kd
• Concentration 1.0mg/ml
• Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB dilution: 1:1000~1:2000
  IP dilution: 1:200
  IHC dilution:1:50-300
  IF dilution:1:200

Cytokeratin17 (CK17) is a protein that in humans is encoded by the KRT17 gene. It is found in nail beds, hair follicles, sebaceous glands, and other epidermal appendages. Mutations in the gene encoding this protein lead to Jackson-Lawler type pachyonychia congenita and steatocystoma multiplex.

• Western blot analysis of 1) Hela, 2) MCF7, 3) 293T, using #38038 diluted at 1:2,000.
• 1¡¢Input: Hela Cell Lysate
  2¡¢IP product: IP dilute 1:200
  Western blot analysis: primary antibody : #38038 1:1,000
  Secondary antibody: Goat anti-Mouse IgG, Light chain specific, 1:5,000
• Immunofluorescence analysis of Human-breast tissue. 1,CK17 Monoclonal Antibody(10A1)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
• Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,CK17 Monoclonal Antibody(10A1) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting