• Product Namealpha Lactalbumin Mouse Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• PurificationAffinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHu B
• Specificityalpha Lactalbumin Mouse Monoclonal antibody detects endogenous alpha Lactalbumin protein.s
• Target Namealpha Lactalbumin
• Alternative Names    Lactose synthase B protein Lysozyme-like protein 7
• Accession No. Swiss-Prot#P00709
• UniprotP00709
• Gene ID3906;
• SDS-PAGE MW 16kd
• Concentration 1.0mg/ml
• Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB dilution: 1:1000~1:3000

  
  IHC dilution:1:50-300
  IF dilution:1:200

In primates, alpha-lactalbumin expression is upregulated in response to the hormone prolactin and increases the production of lactose.α-Lactalbumin forms the regulatory subunit of the lactose synthase (LS) heterodimer and β-1,4-galactosyltransferase (beta4Gal-T1) forms the catalytic component.

• Western blot analysis of 1) Human Milk, 2) Milk, using #38041 diluted at 1:3,000.
• Immunofluorescence analysis of Human-breast tissue. 1,alpha Lactalbumin Monoclonal Antibody(9E9)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
• Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,alpha Lactalbumin Monoclonal Antibody(9E9) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting