• Product NameCD16 Mouse Monoclonal Antibody (Q32)
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.Q32
• PurificationAffinity purification using immunogen.
• ApplicationsWB,IHC
• Species ReactivityHu
• SpecificityAntibody detects endogenous CD16 protein.
• Target NameCD16
• Alternative Names    CD32; CD32A; CDw32; Fc fragment of IgG, low affinity IIa, receptor (CD32); Fc-gamma RII-a; Fc-gamma-RIIa; FCG2; FCG2A; FcGR; FCGR2; FCGR2A; FCGR2A1; FcRII-a; IGFR2
• Accession No. Swiss-Prot#:P12318
• UniprotP12318
• Gene ID2212;
• SDS-PAGE MW 35kd
• Concentration 1.0mg/ml
• Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB dilution: 1:1000~1:2000
  IHC dilution:1:50-300

CD16 is a low affinity Fc receptor.It is a cluster of differentiation found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages.

• Western blot analysis of 1) Jurkat, 2)K562 CD16 Mouse mAb(Q32) #38047
• Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,CD16 Monoclonal Antibody(Q32) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting