• Product NameHistone H3(Acetyl-Lys27) Rabbit Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAffinity purification using immunogen.
• ApplicationsWB, IHC, IF, IP, ELISA
• Species ReactivityHuman, Mouse, Rat, Monkey, Arabidopsis thaliana
• SpecificityThe Histone H3(Acetyl Lys27) Rabbit Polyclonal Antibody detects endogenous Histone H3 (Acetyl Lys27) protein.
• Immunogen DescriptionA synthetic acetylated peptide corresponding to residues surrounding Lys27 of human histone H3.
• Target NameHistone H3(Acetyl-Lys27)
• ModificationAcetyl
• Alternative Names    H3 histone antibody; HIST1H3A antibody; Histone cluster 1; H3a antibody
• Accession No. Swiss-Prot#:P68431
• UniprotP68431
• Gene ID8350;8351;8352;8353;8354;8355;8356;8357;8358;8968;
• SDS-PAGE MW 17kd
• Concentration 1.0mg/ml
• Formulation PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
• Storage Store at -20˚C

•  WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; IP 1:200; ELISA 1:5000

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.

• Western blot analysis of extracts from Hela cells, untreated (-) or treated with TSA (1μM, 18 hr; +), using #HW077 diluted at 1:1,000.
• Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Histone H3 (Acetyl-Lys27) Antibody. The picture on the right is blocked with the synthesized peptide.
• Immunofluorescence analysis of Hela cell. Histone H3 (Acetyl Lys27) Polyclonal Antibody(red) was diluted at 1:200(4° overnight).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting