• Product NameFlag-tag Mouse Monoclonal Antibody (2C5)
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.2C5
• PurificationAffinity purification using immunogen.
• ApplicationsWB
• SpecificityThe Flag tag antibody can recognize C-terminal, internal, and N-terminal Flag-tag fusion?proteins.
• Target NameFlag-tag
• Concentration 1.0mg/ml
• Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:5000~1:10000

The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.

• Western blot analysis of Recombinant protein, using #T524 diluted at 1) 1:5,000, 2) 1:10,000.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting