• Product NameGAPDH Rabbit Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAffinity purification using immunogen.
• ApplicationsWB,IHC
• Species ReactivityHu Rt Ms Mk Chk Hm Sh Xenopus
• SpecificityAntibody detects endogenous GAPDH protein.
• Target NameGAPDH
• Alternative Names    G3PD; GAPD; MGC88685
• Accession No. Swiss-Prot#:P04406
• UniprotP04406
• Gene ID2597;
• SDS-PAGE MW 37kd
• Concentration 1.0mg/ml
• Formulation IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:5000~1:20000

Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively expressed in almost all tissues at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

• Western blot analysis of 293T (1), Rat brain (2), NIH 3T3(3), Sheep Muscle(4),Rabbit testis(5), using #37985 diluted at 1:20,000.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting