• Product NameHistone H3 Mouse Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• PurificationAffinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHu Rt Ms
• SpecificityThe Histone H3 antibody can detects endogenous Histone H3 protein.
• Target NameHistone H3
• Alternative Names    H3/A; H3FA;H3FB; H3FC
• Accession No. Swiss-Prot#:P68431
• UniprotP68431
• Gene ID8350;8351;8352;8353;8354;8355;8356;8357;8358;8968;
• SDS-PAGE MW 17kd
• Concentration 1.0mg/ml
• Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB dilution: 1:2000~1:5000
  IF dilution: 1:100-500
  IP dilution: 1:200
  IHC dilution:1:50-300

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.

• Western blot analysis of 1) Hela, 2) Raw, 3) Mouse Brain Tissue, 4) Rat Brain Tissue, using #37988 diluted at 1:5,000.
• IF analysis of Hela with DAPI (Left) and #37988(Right) diluted at 1:200.
• 1¡¢Input: Hela Cell Lysate
  2¡¢IP product: IP dilute 1:200
  Western blot analysis: primary antibody : #37988 1:2,000
  Secondary antibody: Goat anti-Mouse IgG(H+L) 1:10,000.
• Immunofluorescence analysis of Human-liver-cancer tissue. 1,Histone H3 Monoclonal Antibody(1G1)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
• Immunofluorescence analysis of Mouse-liver tissue. 1,Histone H3 Monoclonal Antibody(1G1)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
• Immunofluorescence analysis of Rat-liver tissue. 1,Histone H3 Monoclonal Antibody(1G1)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
• Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,Histone H3 Monoclonal Antibody(1G1) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
• Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1,Histone H3 Monoclonal Antibody(1G1) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
• Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1,Histone H3 Monoclonal Antibody(1G1) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting