• Product Nameβ-tubulin Mouse Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• PurificationAffinity purification using immunogen.
• ApplicationsWB,IF
• Species ReactivityHu Rt Ms
• SpecificityTheβ-tubulin antibody can detects endogenousβ-tubulin protein.
• Target Nameβ-tubulin
• Alternative Names    beta 5-tubulin; beta Ib tubulin; beta-4 tubulin; M40; MGC117247; MGC16435; OK/SW-cl.56; TBB5; TUBB; TUBB1; TUBB5
• Accession No. Swiss-Prot#:P07437
• UniprotP07437
• Gene ID203068;
• SDS-PAGE MW 55kd
• Concentration 1.0mg/ml
• Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB dilution: 1:5000~1:10000
  
  IF dilution:1:200

Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Tubulin may not be stable in certain cells. For example, expression of β-Tubulin in adipose tissue is very low and therefore β-Tubulin should not be used as loading control for these tissues.

• Western blot analysis of 1) Hela, 2) Mouse Brain tissue, 3) Rat Brain tissue, using #38071 diluted at 1:5,000.
• IF analysis of Hela with antibody (Left) and DAPI (Right) diluted at 1:100.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting