• Product Nameβ II tubulin Mouse Monoclonal Antibody (Rodent Preferred)
• Host SpeciesMouse
• ClonalityMonoclonal
• PurificationAffinity purification using immunogen.
• ApplicationsWB,IHC
• Species ReactivityRt Ms
• SpecificityThe β II tubulin Mouse Monoclonal Antibody detects endogenous β II tubulin protein.
• Target Nameβ II tubulin
• Accession No. Swiss-Prot:Q13885
  Gene ID:7280
• UniprotQ13885
• Gene ID7280;
• SDS-PAGE MW 50kd
• Concentration 1.0mg/ml
• Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB dilution: 1:50000~1:100000
  IHC dilution:1:50-300
  IP dilution: 1:200

Tubulin is the major constituent of microtubules. It exists as a heterodimer consiting of an alpha and a beta subunit. Class II beta tubulin is the major form of tubulin beta in neurons, although it is not neuronal specific. It is found in many other tissues including lung tissue and Schwann cells.

• Western blot analysis of 1) Mouse Brain tissue, 2) Rat Brain tissue, using #38092 diluted at 1:100,000.
• 1¡¢Input: Mouse Brain Tissue Lysate
  2¡¢IP product: IP dilute 1:200
  Western blot analysis: primary antibody : #38092 1:10,000
  Secondary antibody: Goat anti-Mouse IgG, Light chain specific, 1:5,000
• Immunohistochemical analysis of paraffin-embedded Human-colon tissue. 1, II tubulin Monoclonal Antibody(Mix) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
• Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1, II tubulin Monoclonal Antibody(Mix) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
• Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1, II tubulin Monoclonal Antibody(Mix) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting