• Product NameMAPKAPK2 (Phospho-Thr334) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of MAPKAPK2 only when phosphorylated at threonine 334.
• Immunogen Typepeptide
• Immunogen DescriptionPeptide sequence around phosphorylation site of threonine 334 (P-Q-T(p)-P-L) derived from Human MAPKAPK2.
• Target NameMAPKAPK2
• ModificationPhospho
• Alternative Names    EC 2.7.11.1; MAP kinase-activated protein kinase 2; MAPK-activated protein kinase 2; MAPK2; MAPKAP kinase 2; MAPKAPK-2; MAPKAPK2; RPS6KC1; kinase MAPKAPK2
• Accession No. Swiss-Prot#:P49137;
  NCBI Gene#:9261
• UniprotP49137
• Gene ID9261;
• SDS-PAGE MW 49kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Stress-activated serine/threonine-protein kinase involved in cytokines production, endocytosis, reorganization of the cytoskeleton, cell migration, cell cycle control, chromatin remodeling, DNA damage response and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. Phosphorylates ALOX5, CDC25B, CDC25C, ELAVL1, HNRNPA0, HSF1, HSP27/HSPB1, KRT18, KRT20, LIMK1, LSP1, PABPC1, PARN, PDE4A, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to dissociate HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impair their chaperone activities and ability to protect against oxidative stress effectively.

• Western blot analysis of extracts from NIH/3T3 cells, using MAPKAPK2 (Phospho-Thr334) antibody #12103. The lane on the right is treated with the synthesized peptide.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MAPKAPK2 (Phospho-Thr334) antibody #12103. The picture on the right is treated with the synthesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting