• Product NameRB(Phospho-Ser811) antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB
• Species ReactivityHuman
• SpecificityThe antibody detects endogenous level of RB only when phosphorylated at serine 811.
• Immunogen TypePeptide
• Immunogen DescriptionA synthetic phosphorylated peptide around S811 of human RB (NP_000312.2).
• Target NameRB
• ModificationPhospho
• Alternative Names    OSRC;PPP1R130;RB;p105-Rb;pRb;pp110;RB1;Rb
• Accession No. Uniprot:P06400
  GeneID:5925
• UniprotP06400
• Gene ID5925
• SDS-PAGE MW 106kDa
• Concentration 1.0mg/ml
• Formulation PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Storage Store at -20˚C. Avoid freeze / thaw cycles.

• WB 1:1000 - 1:2000

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.

• Western blot analysis of extracts of HeLa cells, using Phospho-RB-S811 antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting