• Product NameESPL1(Phospho-S1126/801) antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB,IF
• Species ReactivityHuman
• SpecificityThe antibody detects endogenous level of ESPL1 only when phosphorylated at serine 1126 and 801.
• Immunogen TypePeptide
• Immunogen DescriptionA phospho specific peptide corresponding to residues surrounding S1126 and S801 of human ESPL1.
• Target NameESPL1
• ModificationPhospho
• Alternative Names    ESP1; SEPA
• Accession No. Swiss-Prot#: Q14674
  NCBI Gene ID: 9700
• UniprotQ14674
• Gene ID9700;
• SDS-PAGE MW 233kd
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IF 1:20 - 1:100

Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids.

• Immunofluorescence analysis of U2OS cells using Phospho-ESPL1-S1126 . Blue: DAPI for nuclear staining.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting