• Product NameMC5R antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total MC5R protein.
• Immunogen TypePeptide
• Immunogen DescriptionA synthetic peptide of human MC5R.
• Target NameMC5R
• Alternative Names    MC2;
• Accession No. Swiss-Prot#: P33032
  NCBI Gene ID: 4161
• UniprotP33032
• Gene ID4161;
• Calculated MW 40kD
• SDS-PAGE MW 37kd
• Concentration 1.0mg/ml
• Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:50 - 1:200

This gene encodes a member of the seven-pass transmembrane G protein-coupled melanocortin receptor protein family that stimulate cAMP signal transduction. The encoded protein is a receptor for melanocyte-stimulating hormone and adrenocorticotropic hormone and is suggested to play a role in sebum generation. [provided by RefSeq, Jun 2010],

• Western Blot analysis of 3T3 cells using MC5-R Polyclonal Antibody
• Western blot analysis of the lysates from HeLa cells using MC5R antibody.
• Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
• Immunofluorescence analysis of MCF7 cells, using MC5R Antibody. The picture on the right is blocked with the synthesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting