• Product NameCytochrome C Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total Cytochrome C protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.71-75(N-P-K-K-Y) derived from Human Cytochrome C.
• Target NameCytochrome C
• Alternative Names    CYC, HCS, THC4,
• Accession No. Swiss-Prot:P99999
  Gene ID:54205
• UniprotP99999
• Gene ID54205;
• Concentration 1.0mg/ml

• Predicted MW: 14kd
  Western blotting: 1: 500~1: 1000

Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.

• Western blot analysis of extracts from 3T3, C6 and A549 cells using Cytochrome C Antibody #21680.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting