• Product NamePaxillin(Phospho-Tyr118) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of Paxillin only when phosphorylated at tyrosine 118.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 118 (H-V-Y(p)-S-F) derived from Human Paxillin.
• Target NamePaxillin
• ModificationPhospho
• Alternative Names    PAXI; PXN;
• Accession No. Swiss-Prot: P49023
  NCBI Protein: NP_001074324.1
• UniprotP49023
• Gene ID5829;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 68kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  

Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).

Tao WA, et al.(2005)Nat Methods.2(8): 591-598.
Zhang Y, et al.(2005) Mol Cell Proteomics.4(9): 1240-1250.
Sanders MA, et al.(2005)J Biol Chem.280(25): 23516-23522.
Rush J, et al.(2004) Nat Biotechnol. 23(1): 94-101.

• Western blot analysis of extracts from 3T3 cells untreated or treated with serum using Paxillin(Phospho-Tyr118) Antibody #11089.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Paxillin(Phospho-Tyr118) Antibody #11089(left) or the same antibody preincubated with blocking peptide(right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting