• Product Namebeta-glucosidase antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationPurified by antigen-affinity chromatography.
• ApplicationsWB IHC IF
• Species ReactivityHu
• Immunogen TypeRecombinant protein
• Immunogen DescriptionRecombinant protein fragment contain a sequence corresponding to a region within amino acids 352 and 529 of Human GBA
• Target Namebeta-glucosidase
• Accession No. Swiss-Prot:P04062
  Gene ID:2629
• UniprotP04062
• Gene ID2629;
• Concentration 0.8mg/ml
• Formulation Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

• Predicted MW: 60kd
  Western blotting: 1:500-1:3000
  Immunohistochemistry: 1:100-1:250
  Immunofluorescence: 1:100-1:200
  

This gene encodes a lysosomal membrane protein that cleaves the beta-glucosidic linkage of glycosylceramide, an intermediate in glycolipid metabolism. Mutations in this gene cause Gaucher disease, a lysosomal storage disease characterized by an accumulation of glucocerebrosides. A related pseudogene is approximately 12 kb downstream of this gene on chromosome 1. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq]

• Sample(30 ug of whole cell lysate)
  A: Hep G2
  7.5% SDS PAGE
  Primary antibody diluted at 1: 500
• Immunohistochemical analysis of paraffin-embedded Lung CA, using GBA antibody at 1: 100 dilution.
• Immunofluorescence analysis of methanol-fixed HeLa, using GBA antibody at 1: 100 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting