• Product NameAKT1S1 antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total AKT1S1 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human AKT1S1.
• Target NameAKT1S1
• Alternative Names    Lobe; PRAS40;
• Accession No. Swiss-Prot#: Q96B36
  NCBI Gene ID: 84335
• UniprotQ96B36
• Gene ID84335;
• SDS-PAGE MW 27kd
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:100
  IF 1:50 - 1:100

AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003

• Western blot analysis of extracts of various cell lines, using AKT1S1 at 1:1000 dilution.
• Immunohistochemistry of paraffin-embedded human mammary cancer using AKT1S1 at dilution of 1:200 (40x lens).
• Immunohistochemistry of paraffin-embedded human stomach using AKT1S1 at dilution of 1:200 (40x lens).
• Immunofluorescence analysis of NIH-3T3 cells using AKT1S1 Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
• Western blotting analysis using AKT1S1 antibody #38768.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting